|
| Status |
Public on Nov 18, 2009 |
| Title |
Tatematsu_2-4_axillary-shoots-at-24-h-after-decapitation_Rep2_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Col-0
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Col-0
tissue: axillary shoots
|
| Growth protocol |
Plants were grown under continuous light at 22°C. Nonsterilized plants were grown in pots containing a 1:1 mixture of vermiculite:Jiffy mix (Sakatanotane).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit protocol
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in Affymetrix manual "3' IVT Express Kit User Manual"
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
Growth Room Commercial soil 22ºC 55%
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Nov 12, 2009 |
| Last update date |
Nov 17, 2009 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE18987 |
Transcriptome analysis during axillary shoot outgrowth |
|
