|
| Status |
Public on Nov 18, 2009 |
| Title |
Synek_1-4_exo70A1-2_Rep2_ATH1 |
| Sample type |
RNA |
| |
|
| Source name |
Col-0
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Col-0
developmental stage: 1.0 week
tissue: whole plant
genome/variation: exo70A1 mutant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit protocol
|
| Label |
biotin
|
| Label protocol |
Labelled using protocol described in Affymetrix manual "3' IVT Express Kit User Manual"
|
| |
|
| Hybridization protocol |
Biotin Labelled cRNA using "Affymetrix GeneChip Hybridization, Wash and Stain Kit", according to guidelines set out by Affymetrix, Santa Clara, CA
|
| Scan protocol |
Scanned according to guidelines set out by Affymetrix, Santa Clara, CA.
|
| Description |
Sterilized seeds are stored for 3 days in water at 4ºC. 3 min in 70% ethanol; 2x 5 min in 10% commercial bleach under shaking; 3x rinse in sterile distilled water Seedlings were grown vertically on 1.6% agar in square plates (12x12 cm). Plants were grown in a climate chamber at 22ºC under constant light. 1.6 75 seeds per plate (12x12 cm) in 3 rows 22 ºC average, 22 ºC day, 22 ºC night 65 % average, 65 % day, 65 % night Murashige & Skoog basal salt mixture Constant light
|
| Data processing |
Raw data from the microarrays was normalized at probe-level using MAS5 algorithm. The detection calls (present, marginal, absent) for each probe set was obtained using the GCOS system
|
| |
|
| Submission date |
Nov 12, 2009 |
| Last update date |
Nov 17, 2009 |
| Contact name |
Nottingham Arabidopsis Stock Centre (NASC) |
| E-mail(s) |
[email protected]
|
| Phone |
+44 (0)115 951 3237
|
| Fax |
+44 (0)115 951 3297
|
| URL |
http://arabidopsis.info/
|
| Organization name |
Nottingham Arabidopsis Stock Centre (NASC)
|
| Department |
School of Biosciences, University of Nottingham
|
| Street address |
Sutton Bonington Campus
|
| City |
Loughborough |
| ZIP/Postal code |
LE12 5RD |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE18986 |
Transcriptome of the exo70A1 mutant |
|
