|
| Status |
Public on Jan 19, 2010 |
| Title |
rectus_abdomins_UGI_cancer_13 |
| Sample type |
RNA |
| |
|
| Source name |
rectus abdomins muscle UGI cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: rectus abdominus
disease status: upper GI cancer
weightloss: 2.37113402061855
crp: 1
age (years): 66
gender: Male
body mass index (bmi): 30.3990883483537
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle was homogenized in Trizol with a Mini-Beadbeater-8 homogenizer and RNA extraction carried out using Trizol protocol (Invitrogen). Hybridization, washing, staining and scanning of the arrays were performed according to manufacturer's instructions (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
As per standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
As per standard Affymetrix protocol.
|
| Scan protocol |
Scanning was carried out according to Affymetrix directions.
|
| Description |
F52.CEL
|
| Data processing |
All arrays were processed using the Microarray Suite version 5.0 (MAS 5.0) algorithm to a global scaling intensity of 100. Data were filtered using present/absent calls. Probes called absent by MAS 5.0 across all arrays were discarded. Normalization was carried out using RMA in the bioconductor suite (www.bioconductor.org) version 2.1.
|
| |
|
| Submission date |
Oct 30, 2009 |
| Last update date |
Jan 19, 2010 |
| Contact name |
Iain Gallagher |
| E-mail(s) |
[email protected]
|
| Phone |
00 44 1786 46 6024
|
| Organization name |
University of Stirling
|
| Department |
Faculty of Health Sciences and Sport
|
| Street address |
Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
|
| City |
Stirling |
| ZIP/Postal code |
FK9 4LA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL9486 |
| Series (1) |
| GSE18832 |
mRNA profiling in rectus abdominis muscle from patients with upper GI cancer |
|
