|
| Status |
Public on Oct 23, 2009 |
| Title |
SKP sample 5 |
| Sample type |
RNA |
| |
|
| Source name |
skin-derived precursor cells
|
| Organism |
Mus musculus |
| Characteristics |
passage: primary
cell type: skin-derived precursor cells
|
| Growth protocol |
Neonatal back skin (P4) from Sox2:EGFP mice was processed as described in the paper to yield a single cell suspension. Cells were stained with biotin conjugated anti-cd34 antibody and visualized with a PECy7 conjugated streptavidin secondary antibody. Sox2:EGFP-positive, cd34-negative cells were sorted directly into RNAlater (Ambion). For RNA isolation of SKPs, primary SKPs were generated from neonatal (P4) mouse skin as described in the paper. Spherical SKP colonies were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix) and scanned at the HSC Microarray Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
SKP colonies
|
| Data processing |
The data were background corrected and normalized using standard RMA procedure implemented in the Affymetrix Expression Console software. The preprocessed data were analysed using the LIMMA Bioconductor package to identify genes that show significant evidence of differential expression between SKPs and Sox:EGFP-positive cells.
|
| |
|
| Submission date |
Oct 22, 2009 |
| Last update date |
Oct 22, 2009 |
| Contact name |
Freda Miller |
| E-mail(s) |
[email protected]
|
| Organization name |
Hospital for Sick Children
|
| Street address |
555 University Avenue
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE18690 |
SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells |
|
