|
| Status |
Public on Jan 31, 2010 |
| Title |
YAC128 mouse, 12 months of age (S1bis) |
| Sample type |
RNA |
| |
|
| Source name |
Mouse id 53.107.121
|
| Organism |
Mus musculus |
| Characteristics |
age: 12 months
tissue: whole striatum
genotype: YAC128
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Striatal tissue was collected from 12 and 24 month old YAC128 mice and wildtype littermates. Animals were anaesthetized using Avertine and tissue was collected in RNAlater (Ambion) and stored at -80 prior to RNA isolation. Homogenization of tissue was performed using a Fastprep Homogenizer (ThermoScientific). Total RNA from mouse striatum was extracted using the Qiagen RNeasy mini kit and eluted in 30 microliters RNase free water according to manufacturer's instructions. Total RNA was quantified using the Nanodrop spectrophotometer (ThermoScientific) and the integrity of the total RNA was determined electrophoretically on the RNA Nano Assay Chip run on the Bioanalyzer 2100 (Agilent Tech).
|
| Label |
biotin
|
| Label protocol |
RNA was prepared according to the manufacturer.s two-cycle target labeling procedure (Affymetrix Inc.) with a starting amount 25 ng total RNA per sample.
|
| |
|
| Hybridization protocol |
According to the manufacturer's instructions.
|
| Scan protocol |
According to the manufacturer's instructions.
|
| Description |
n/a
|
| Data processing |
All CEL files in the series were processed using RMA in R using justRMA with default parameters.
|
| |
|
| Submission date |
Oct 14, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Paul Pavlidis |
| E-mail(s) |
[email protected]
|
| Organization name |
University of British Columbia
|
| Department |
Centre for High-Throughput Biology / Psychiatry
|
| Street address |
177 Michael Smith Laboratories 2185 East Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE18551 |
Striatum of Huntington's disease model mice [Affymetrix data] |
| GSE19677 |
Striatum of Huntington's disease model mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
