|
| Status |
Public on Dec 01, 2021 |
| Title |
mammary tumor tissue [L0] |
| Sample type |
RNA |
| |
|
| Source name |
mammary mTDT, BALB/c host
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mammary mTDT
immune infiltrate: inflamed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen), according to manufacturer's instructions.
|
| Label |
Nanostring bar coding
|
| Label protocol |
Specimens were labeled with decode assignment which was linked to tumor origin.
|
| |
|
| Hybridization protocol |
100 ng RNA was hybridized to NanoString® PanCancer IO360™ panel code set according to the recommendations of the manufacturer (NanoString Technologies, Inc., Seattle, Washington, USA).
|
| Scan protocol |
Following hybridization, the target-probe complexes were purified and immobilized on the nCounter prep station. Digital counts for each gene-specific target RNA were then acquired on the nCounter detection analyzer and normalized to account for differences in tissue mRNA quantity as well as slight differences in assay efficiency.
|
| Description |
L0
|
| Data processing |
The nSolver 2.6 software was used to normalize expression values using housekeeping genes following the manufacturer's recommendations.
|
| |
|
| Submission date |
Jun 12, 2020 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Jade Moore |
| Organization name |
University of California San Francisco
|
| Street address |
2340 Sutter St, S371
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94115 |
| Country |
USA |
| |
|
| Platform ID |
GPL28689 |
| Series (1) |
| GSE152403 |
Mammary Tumor-Derived Transplants as Breast Cancer Models to Evaluate Tumor-Immune Interactions and Therapeutic Responses |
|
