3J - time course study - pathogen infection, ralstonia solanacearum gmi 1000 strain: quantity 1e+07cfu/ml time 72h. Inoculation was performed as described (Vailleau et al., 2007). Bacterial concentration was set at 107cfu/ml for the inoculation.
Growth protocol
root - Seeds were surface sterilized, vernalized and germinated according to Boisson-Dernier (Boisson-Dernier et al., 2001). 24h germinating seed were grown on Fahraeus medium (Fahraeus, 1957) covered with filter paper in square Petri dishes at 25°C (day/night 12h). Roots kept in the dark with a mask on the Petri dish made of kraft paper and aluminium.
Extracted molecule
total RNA
Extraction protocol
SKL3J_R3:8.6ug.
Label
Biotin
Label protocol
labelling Biotin direct, amplification=yes, cRNA 20 ug.
