strain: Wild-type mouse (Mahapatra et al., 2005) sex: Male age: 12-weeks tissue: Liver
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted from liver from wild-type (n=3) and Chga knockout (n=3) mice with the RNAzol (guanidinium thiocyanate) kit (TelTest, Friendswood, TX), followed by RNase-free DNase I (Qiagen, Valencia, CA) treatment to eliminate residual genomic DNA.
Label
biotin
Label protocol
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse430A_2 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description
Gene expression data from wild-type mouse liver.
Data processing
The data were analyzed with GeneChip Operating Software (GCOS v1.2) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 2500.
