|
| Status |
Public on Mar 03, 2010 |
| Title |
ChgaKO_liver_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Chromogranin A knockout mouse, liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: Chromogranin A knockout mouse (Mahapatra et al., 2005)
sex: Male
age: 12-weeks
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from liver from wild-type (n=3) and Chga knockout (n=3) mice with the RNAzol (guanidinium thiocyanate) kit (TelTest, Friendswood, TX), followed by RNase-free DNase I (Qiagen, Valencia, CA) treatment to eliminate residual genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Mouse430A_2 GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from chromogranin A knockout mouse liver.
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS v1.2) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 2500.
|
| |
|
| Submission date |
Sep 28, 2009 |
| Last update date |
Mar 03, 2010 |
| Contact name |
Ryan Friese |
| E-mail(s) |
[email protected]
|
| Phone |
858 552-8585 ext. 2619
|
| Fax |
858 552-8585 ext. 2619
|
| Organization name |
University of California San Diego
|
| Department |
|
| Street address |
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE18305 |
Liver gene expression from chromogranin A knockout mice (Mahapatra et al. 2005) vs. wild-type mice |
| GSE18332 |
Gene expression from chromogranin A knockout mice vs. wild-type mice |
|
