|
| Status |
Public on Jul 30, 2020 |
| Title |
CD8_KO_2 |
| Sample type |
RNA |
| |
|
| Source name |
CD8+ T cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Dot1l knockout
cell type: CD8+ T cell
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Dot1l flox/flox mice were crossed with CD4-Cre mice. Mice were housed and bred in a specific pathogen free (SPF) animal facility. Fresh CD8+ T cells in the peripheral lymph nodes and spleen from Dot1l knockout and wild type mice were isolated by EasySep™ Mouse CD8+ T Cell Isolation Kit (STEMCELL, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from these cells using Direct-zolTM RNA miniprep Plus kit (ZOMO research, Irvine, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2) from 400 ng total RNA.
|
| |
|
| Hybridization protocol |
Following labeling procedure, 2.76 ug of cDNA were hybridized at 48C on a Mouse Gene ST 2.1 Array, and washed and stained using the Affymetrix Gene Atlas system (software version 2.0.0.460).
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system (software version 2.0.0.460).
|
| Data processing |
RMA was used to fit log2 expression values to the data using the oligo bioconductor package in R version 3.1.1.
|
| |
|
| Submission date |
May 19, 2020 |
| Last update date |
Jul 30, 2020 |
| Contact name |
Yingjie Bian |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Department |
Surgery
|
| Street address |
109 Zina Pitcher Pl
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE150845 |
RNA-array in DOT1L knockout and wild type CD8+ T cells |
| GSE150887 |
Cancer SLC43A2 alters T cell methionine metabolism and histone methylation |
|
