|
| Status |
Public on Dec 15, 2009 |
| Title |
Air 90 |
| Sample type |
RNA |
| |
|
| Source name |
Air 90
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
exposure type: Humidified air
minutes after exposure: 60
gender: male
|
| Treatment protocol |
Flies were either untreated (2 replicates), or treated with 30 min ethanol vapor or humidified air. RNA was extracted 0, 30, 60, 90, 120, 180, or 210 min following exposure termination, and were snap frozen in liquid nitrogen.
|
| Growth protocol |
200 2-4 day old male flies from synchronized cultures were used for all conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Trizol reagent
|
| Label |
biotin
|
| Label protocol |
single labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
Head RNA extracted 60' after Drosophila exposed for 30' to humidified air.
|
| Data processing |
R/Bioconductor GCRMA
|
| |
|
| Submission date |
Sep 22, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Fred Wolf |
| E-mail(s) |
[email protected]
|
| Organization name |
Ernest Gallo Clinic and Research Center
|
| Street address |
5858 Horton St Suite 200
|
| City |
Emeryville |
| State/province |
CA |
| ZIP/Postal code |
94608 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE18208 |
Acute ethanol exposure time-course in Drosophila melanogaster |
|
| Relations |
| Reanalyzed by |
GSE119084 |
