|
| Status |
Public on Jul 19, 2010 |
| Title |
Strain a14 replicate 1 (one color) |
| Sample type |
genomic |
| |
|
| Source name |
DNA from Strain_a14
|
| Organism |
Neisseria meningitidis |
| Characteristics |
strain: a14
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromosomal DNA from the meningococcal strains were isolated using Genomic Tip 20/G (Qiagen, Germany).
|
| Label |
Cy3
|
| Label protocol |
4 µg of chromosomal DNA with Cy3 dCTP using Klenow enzyme and random nonamers and the labelled DNA was precipitated and purified.
|
| |
|
| Hybridization protocol |
The labelled DNA were mixed together with two volumes of hybridization buffer (Eurogentec, Belgium) and hybridized manually at 50°C
|
| Scan protocol |
The slides were scanned using a Scanarray HT scanner(Perkin Elmer) with settings to avoid more than 10% of the spots being saturated.
|
| Description |
DNA isolated from the strain using Genomic Tip DNA isolation kit from Qiagen
|
| Data processing |
Raw data generated after flagging for obvious cases of stray background and spots with a signal to noise ratio of less than 3 were further analyses using the R package limma.
|
| |
|
| Submission date |
Sep 17, 2009 |
| Last update date |
Jul 19, 2010 |
| Contact name |
Biju Joseph Ampattu |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Wuerzburg
|
| Department |
Department of Microbiology
|
| Street address |
Josef-Schneider Str 2
|
| City |
Wuerzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
| |
|
| Platform ID |
GPL9218 |
| Series (1) |
| GSE18159 |
Comparative genome hybridization of meningococcal isolates using 1 color microarray |
|
