|
| Status |
Public on Sep 19, 2009 |
| Title |
Cbfb-deficient Treg_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Cbfb-deficient Treg
|
| Organism |
Mus musculus |
| Characteristics |
cell type: peripheral CD4+CD25hi cells
mouse genotype: Cbfb-flox/flox, Foxp3-ires-Cre
gender: male
age: 16 weeks
tissue: purified CD4+CD25hi cells from spleen and lymph nodes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy Micro Kit (QIAGEN) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
n/a
|
| Data processing |
Data analyses were done with the use of MeV (v4.2) (Saeed et al., 2003 Biotechniques 34, 374-378).
|
| |
|
| Submission date |
Sep 17, 2009 |
| Last update date |
Sep 21, 2009 |
| Contact name |
Akihiko Kitoh |
| Organization name |
Kyoto University
|
| Department |
Institute for Frontier Medical Sciences
|
| Lab |
Department of Experimental Pathology
|
| Street address |
53 Shogoin Kawahara-cho
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18148 |
Microarray analysis of Cbfb-deficient regulatory T cells |
|
