|
| Status |
Public on May 06, 2020 |
| Title |
KO control [3814] |
| Sample type |
RNA |
| |
|
| Source name |
kidney
|
| Organism |
Mus musculus |
| Characteristics |
tissue: kidney
genotype: IL-33 KO
treatment: control
|
| Treatment protocol |
Unilateral ureteral obstruction in IL-33-deficient and wild type C57/BL6 mice, tissue harvest 24h after ligation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from tissue homogenates was isolated with the RNeasy Mini Kit according to the manufacturer’s instructions (Qiagen). RNA purification was performed using an RNase-Free DNAse Set (Qiagen¨)
|
| Label |
biotin
|
| Label protocol |
Illumina Total Prep 96 RNA Amp Kit
|
| |
|
| Hybridization protocol |
750 ng of biotin-labeled cRNA was hybridized to the Illumina MouseRef-8 v2 Expression BeadChip
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The raw data was pre-processed by Illumina´s BeadStudio software V2
Data were normalized by Quantile Normalization of micrarraoy data, please refer to below paper (https://academic.oup.com/bioinformatics/article/19/2/185/372664)
|
| |
|
| Submission date |
May 05, 2020 |
| Last update date |
May 06, 2020 |
| Contact name |
Guttorm Haraldsen |
| E-mail(s) |
[email protected]
|
| Phone |
+4792401962
|
| Organization name |
Oslo University Hospital
|
| Department |
Pathology
|
| Lab |
Haraldsen-lab
|
| Street address |
Sognsvannsveien 20
|
| City |
Oslo |
| ZIP/Postal code |
0027 |
| Country |
Norway |
| |
|
| Platform ID |
GPL15097 |
| Series (2) |
| GSE149915 |
Unilateral ureteral obstruction in IL-33-deficient and wild type C57/BL6 mice |
| GSE149918 |
Interleukin-33 in experimental kidney fibrosis |
|
