|
| Status |
Public on Jan 01, 2012 |
| Title |
htHSC sample 2 replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Sca-1+,Lin-,AA4.1+,c-Kit++ FLHSC from 15 embryos combined
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6
genotype: Crebbp+/-
cell type: Sca-1+,Lin-,AA4.1+,c-Kit++ FLHSC
age: 14 dpc
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy® micro kit following manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
12ng of total RNA was amplified following the Ovation RNA amplification protocol.
12 nanograms of total RNA was amplified and processed to produce biotinylated cDNA targets using the NuGen Ovation Biotin labeling system.
|
| |
|
| Hybridization protocol |
3.75 micrograms of biotinylated material was hybridized according to the standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
14.5dpc fetal liver HSC
|
| Data processing |
GCRMA probe summarization and normalization using the Bioconductor gcrma package (http://www.bioconductor.org).
|
| |
|
| Submission date |
Sep 10, 2009 |
| Last update date |
Jan 01, 2012 |
| Contact name |
Madeleine E. Lemieux |
| E-mail(s) |
[email protected]
|
| Phone |
617-595-6695
|
| URL |
http://www.bioinfo.ca
|
| Organization name |
Bioinfo
|
| Street address |
275 Main St., Suite 252
|
| City |
Plantagenet |
| State/province |
ON |
| ZIP/Postal code |
K0B 1L0 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18061 |
MDS and DNA repair defects in Crebbp+/- mice |
|
