|
| Status |
Public on Sep 30, 2020 |
| Title |
HC_2 |
| Sample type |
SRA |
| |
|
| Source name |
healthy_peripheral blood B cells
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: healthy control adult from US
subject id: control 2
age: 29 years
Sex: Female
tissue: Blood
cell type: Peripheral mononuclear blood cells (PBMCs)
|
| Growth protocol |
Cell culture protocol: Peripheral blood draw was done, and mononuclear cells were isolated by ficoll gradient purification. B cells were isolated with the EasySep Human B cell isolation kit on a RoboSep platform
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were encapsulated in droplets using the Chromium Controller (10X Genomics). Reverse transcription and cDNA amplification were performed using the Chromium Single Cell 3’ kit (10X Genomics). All steps were done according to manufacturer’s instructions. 100 ng of cDNA (25% of generated cDNA) was used as input for library preparation.
Libraries were sequenced using the Illumina Hiseq3000 platform 8 lanes of sequencing at 26bp-8bp-98bp according to 10X Genomics sequencing recommendations.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
The sample replicates were split into 8 Hiseq 2500 sequencing lanes and for each replicate, the lanes were merged into a single count matrix using Cell Ranger v3.0 (10X Genomics). Further, the resultant counts matrices for each replicate were processed using an inhouse single-cell RNA-seq pipeline that uses Seurat v 3.0
Genome_build: Human - hg38
Supplementary_files_format_and_content: CSV files are generated from CellrangerV3.0 and Integrated matrix file is generated by integrating the samples from healthy controls and Malaria patients using SeuratV3.0
|
| |
|
| Submission date |
May 01, 2020 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Arun Boddapati |
| E-mail(s) |
[email protected]
|
| Organization name |
Yerkes National Primate research center
|
| Department |
Genomics Core
|
| Street address |
954 Gatewood Road, NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329 |
| Country |
USA |
| |
|
| Platform ID |
GPL27467 |
| Series (2) |
| GSE149728 |
Chronic Malaria drives functional heterogenity in B cell subpopilations and expansion of unswitched atypical memory B cells [Malaria scRNA-seq] |
| GSE149729 |
Chronic Malaria drives functional heterogenity in B cell subpopilations and expansion of unswitched atypical memory B cells |
|
| Relations |
| BioSample |
SAMN14791582 |
