|
| Status |
Public on May 17, 2010 |
| Title |
P19_differentiation_10day_repA |
| Sample type |
RNA |
| |
|
| Source name |
P19CL6 cells, DMSO differentiation 10 days
|
| Organism |
Mus musculus |
| Characteristics |
cell line: P19CL6
|
| Treatment protocol |
P19CL6 cells are differentiated by plating at a density of 3.7 x 10E5 cells/10cm dish in differentiation medium containing alpha-MEM, 10% FBS, P/S and 1% DMSO, followed by new differentiation medium every 48 hours.
|
| Growth protocol |
P19CL6 cells are maintained in undifferentiated state through frequent (q 48 hr) passage in alpha-MEM plus 10% ES cell grade FBS and P/S at 1:20.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared from cultures by Trizol extraction and purification with the RNeasy Mini kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Total RNA (2 micrograms) was converted into biotin-labeled cRNA using the SuperScript II cDNA synthesis kit (Invitrogen, Carlsbad, CA) and the Enzo HighYield in vitro transcription amplification kit (Enzo Life Sciences, Farmingdale, NY).
|
| |
|
| Hybridization protocol |
Samples were hybridized overnight to GeneChips in accordance with Affymetrix protocols.
|
| Scan protocol |
Post hybridization washing, staining and fluorescence scanning were performed using Affymetrix instrumentation in accordance with manufacturer recommendations.
|
| Description |
P19CL6 cells, DMSO differentiation 10 days
|
| Data processing |
P19CL6 sample microarray data were normalized using the ArrayQuest webtool (Argraves et al., 2005) to implement the Bioconductor (Gentleman et al., 2004) build of GCRMA (Wu et al., 2003).
|
| |
|
| Submission date |
Sep 01, 2009 |
| Last update date |
May 17, 2010 |
| Contact name |
Jeremy Barth |
| E-mail(s) |
[email protected]
|
| Organization name |
MUSC
|
| Street address |
173 Ashley Ave, BSB 627
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29425-5080 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE17910 |
In vitro differentiation of P19CL6 cardiogenic embryonic carcinoma cells |
| GSE17936 |
Nkx2.5 regulates Jarid2 during outflow tract morphogenesis |
|
