|
| Status |
Public on Jan 01, 2022 |
| Title |
WT2U_ZT0 |
| Sample type |
SRA |
| |
|
| Source name |
fly heads
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: Canton-S w1118 (iso1CJ)
age: 27 days old
timepoint: Zeitgeber time 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagents and homogenized using a BeadBug microtube homogenizer (Benchmark Scientific). Samples were further extracted using chloroform and the aqueous phase containing nucleic acid was collected. RNeasy Mini Kit (Qiagen) was then used to remove DNA with DNase and further purify the samples, following the manufacturer's protocol.
Sequencing libraries were prepared with the Illumina TruSeq stranded mRNA LT kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Raw_data_combined.txt
|
| Data processing |
bcl2fastq v2.20 was used for basecalling.
STAR v2.4.2a was used for alignment.
featureCounts was used for generate counts matrix.
Genome_build: FlyBase release 6.13 (May 2016)
Supplementary_files_format_and_content: tab-delimited text files include raw counts for each aligned gene for each sample
|
| |
|
| Submission date |
Apr 06, 2020 |
| Last update date |
Jan 01, 2022 |
| Contact name |
Zikun Wang |
| E-mail(s) |
[email protected]
|
| Organization name |
The Rockefeller University
|
| Lab |
Young Laboratory
|
| Street address |
1230 York Av
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10044 |
| Country |
USA |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE148136 |
Identification of gene expression changes in sleep mutants associated with reduced longevity in Drosophila |
|
| Relations |
| BioSample |
SAMN14543787 |
| SRA |
SRX8062626 |
