|
| Status |
Public on Feb 01, 2021 |
| Title |
Diabetic_0h_03 |
| Sample type |
RNA |
| |
|
| Source name |
2mm skin biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human skin grafted in diabetic mice
disease state: diabetic
time: 0 hours
|
| Treatment protocol |
Experimental diabetes was induced by intraperitoneal injections of STZ, administered on five consecutive days. Wound healing experiments were performed using a biopsy punch (Pfm Medical Ag.,Cologne, Germany) in the stable human skin engrafted on immunodeficient mice exposed to sustained hyperglycaemia for 6 weeks. Specifically, 2mm-circular excisional wounds were created and the excised tissue was harvested and used as a reference for gene expression analysis
|
| Growth protocol |
Human skin biopsies of healthy donors, isolated by biomechanical and enzimatic digestion and expanded in vitro
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed using Trizol (Invitrogen) extraction based methods, followed by purification in columns (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
generate double-stranded cDNA using the protocol for one-cycle cDNA synthesis. Biotin-labeled cRNA was fragmented and hybridized overnight to Affymetrix HU133 2.0 GeneChip
|
| |
|
| Hybridization protocol |
16h of hybridization at 45ºC,
|
| Scan protocol |
arrays were washed, stained with streptavidin phycoerythrin, and were then scanned with the GeneArray Scanner (Affymetrix) to generate an image file.
|
| Data processing |
PCA on samples detected two outliers (C0_04 and C0_06). Raw data from microarrays excluding outliers was normalized using the rma algorithm from the affy package (Bioconductor)
|
| |
|
| Submission date |
Apr 01, 2020 |
| Last update date |
Feb 01, 2021 |
| Contact name |
carlos leon |
| E-mail(s) |
[email protected]
|
| Phone |
916248211
|
| Organization name |
UC3M
|
| Department |
Bioengineering
|
| Street address |
Avenida Universidad 30
|
| City |
Leganes |
| State/province |
Madrid |
| ZIP/Postal code |
28911 |
| Country |
Spain |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE147890 |
Transcriptomic characterization of the delayed wound healing response in a diabetic skin humanized mice model |
|
