|
| Status |
Public on Aug 22, 2009 |
| Title |
ButMorphine Rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Induction: butyrate-morphine. Challenge: morphine
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Striatum
age: 2 months
gender: male
|
| Treatment protocol |
Behavioural sensitization: Locomotor sensitization induced by morphine was evaluated using a protocol divided into two phases: induction and challenge. The induction phase involved six trials on alternate days, one trial per day. On each one of these trials, mice received and injection of saline or sodium butyrate (300 mg/kg) immediatedly followed by a second injection of morphine (20 mg/kg). The challenge phases consisted of a single trial conducted 7 days after the last test of the induction phase. In this case, all animals received a single injection of morphine (20 mg/kg). Striatal RNA was extracted 1h after the morphine challenge in groups of four animals that received either saline (N=6) or morphine (N=3), or the sodium butyrate-morphine (N=3) co-treatment during the sensitization induction phase.
|
| Growth protocol |
Two months-old Swiss-Albino male mice maintained in housing standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice striatal tissue was rapidly dissected from anterior 6mm coronal sections using a chilled acrylic mouse brain slicer matrix (Zivic Instruments) with 2 mm coronal section slice intervals, in RNAlater solution until further RNA extraction (Qiagen, Venlo, The Netherlands). Equal amounts of total RNA from four animals were pooled, processed and hybridized to Mouse Gene 1.0 ST genechips (Affymetrix, Santa Clara, CA). Three to six biological replicates of the microarray were prepared for each experimental group (saline-saline, N=6; saline-morphine, N=3; butyrate-morphine, N=3)..
|
| Label |
biotin
|
| Label protocol |
Following Affymetrix instructions
|
| |
|
| Hybridization protocol |
Following Affymetrix instructions
|
| Scan protocol |
Following Affymetrix instructions
|
| Description |
Gene expression data from striatal tissue
|
| Data processing |
GeneSpring GX software (Agilent Technologies Inc) was used. Data were extracting using RMA16 algorithm and values were normalized by the median of controls. Data are represented as log2
|
| |
|
| Submission date |
Aug 20, 2009 |
| Last update date |
Sep 16, 2019 |
| Contact name |
Angel Barco |
| Organization name |
Instituto de Neurociencias (UMH-CSIC)
|
| Street address |
Av. Santiago Ramón y Cajal
|
| City |
Sant Joan d'Alacant |
| State/province |
Alicante |
| ZIP/Postal code |
03550 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE17731 |
Selective boosting of transcriptional and behavioral responses to drugs of abuse by histone deacetylase inhibition |
|
