|
| Status |
Public on Oct 01, 2009 |
| Title |
Corticospinal_Motor_Neurons_P3_rep2_430 2.0 array |
| Sample type |
RNA |
| |
|
| Source name |
corticospinal motor neurons, postnatal day 3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue type: neuronal
cell type: corticospinal motor neurons
age: Postnatal day 3
|
| Treatment protocol |
Neurons were dissociated and microsphere-labeled CSMN, CPN, or CTPN were purified by fluorescence-activated cell sorting (FACS) directly into RNAlater.
|
| Growth protocol |
All neuronal subtypes were purified from C57BL/6 mice. Neuronal subtypes were retrogradely labeled with green fluorescent microspheres injected into appropriate axonal fields: sensorimotor cortex (CSMN and CPN) or visual cortex (CTPN).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the StrataPrep Total RNA Micro Kit (Stratagene).
|
| Label |
biotin
|
| Label protocol |
RNA was amplified according to the Affymetrix small sample protocol, using two consecutive rounds of linear in vitro transcription to obtain 15-20 ug of amplified and labeled cRNA for each hybridization.
|
| |
|
| Hybridization protocol |
Standard protocols for Affymetric protocol for 430 2.0 microarrays were used. Arrays were washed on an Affymetrix fluidics station.
|
| Scan protocol |
According to standard Affymetric protocols.
|
| Description |
Cells purified by fluorescence-activated cell sorting from murine (C57BL/6) cortex
|
| Data processing |
RMA normalization was applied using Bioconductor (www.bioconductor.org).
|
| |
|
| Submission date |
Aug 19, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Bradley Molyneaux |
| E-mail(s) |
[email protected]
|
| Phone |
617-726-3552
|
| Organization name |
Harvard Medical School
|
| Department |
MGH-HMS Center For Nervous System Repair
|
| Lab |
Macklis Lab
|
| Street address |
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE17783 |
Analysis of gene expression in FACS-purified cortical projection neurons using Affymetrix 430 2.0 microarrays |
| GSE17784 |
Gene expression in FACS-purified cortical projection neurons |
|
| Relations |
| Reanalyzed by |
GSE119085 |
