|
| Status |
Public on Mar 07, 2016 |
| Title |
HIV positive PD-1 negative case 4 |
| Sample type |
RNA |
| |
|
| Source name |
HIV positive PD-1 negative case 4
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD4+ T cell
hiv rna (cop./ml): < 50
cd4+ t cell count: 550
disease state: HIV positive
pd-1 status: PD-1 negative
|
| Treatment protocol |
CD4+ T cells were not further manipulated
|
| Growth protocol |
CD4+ T cells were isolated from HIV infected individuals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in Trizol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
HIV positive PD-1 negative
|
| Data processing |
The data were normalised using quantile normalisation in R
|
| |
|
| Submission date |
Aug 12, 2009 |
| Last update date |
Mar 07, 2016 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
[email protected]
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6106 |
| Series (2) |
| GSE17606 |
TNF dependent induction of inhibitory pathways in CD4+ T cells in HIV and LCMV [HIV-infected individuals] |
| GSE52185 |
TNF dependent induction of inhibitory pathways in CD4+ T cells in HIV and LCMV |
|
