|
| Status |
Public on Nov 01, 2009 |
| Title |
Wilt type flg22 2h 2nd rep |
| Sample type |
RNA |
| |
|
| Source name |
5weeks old arabidopsis leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: Leaf tissue
|
| Treatment protocol |
Leaves were inoculated with PstDc3000 at 10^6cfu/ml or with water. Tissues were harvested at 24hrs post inoculation
|
| Growth protocol |
Plants were grown in short day environment (8hrs light/16 hrs dark) for 5 weeks at 20C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction according to the manufacturer instruction. The total RNA was then DNAse treated using the Qiagen DNAse. Purification was performed using the RNAeasy columns following the manufacturer instruction
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Arabidopsis Genome ATH1 microarray. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® Scanner 3000
|
| Description |
Genes expression on leaf tissue after flg22 (100nM) treatment
|
| Data processing |
background corrected using RMA, quantile normalized, values summarised by median-polish
|
| |
|
| Submission date |
Aug 03, 2009 |
| Last update date |
Aug 05, 2009 |
| Contact name |
Daniel MacLean |
| E-mail(s) |
[email protected]
|
| Phone |
016033221297
|
| URL |
http://www.tsl.ac.uk
|
| Organization name |
The Sainsbury Laboratory
|
| Department |
Bioinformatics
|
| Street address |
Colney Lane
|
| City |
Norwich |
| ZIP/Postal code |
NR4 7UH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE17479 |
Effect of auxin signaling on flg22 response |
|
