agent: ConA time point: 3 hr replicate: 3 age: 8- to 10-week-old gender: male strain: Balb/c tissue: whole liver
Extracted molecule
total RNA
Extraction protocol
Liver samples from each mouse were ground into a fine powder in a mortar cooled by liquid nitrogen, and 100 mg was added to 1 ml prechilled Trizol reagent (Invitrogen, Carlsbad, CA). Total RNA extractions were performed according to the manufacturer’s directions, and the RNA was further puri-fied by passage through RNeasy mini-columns (QIAGEN, Valencia, CA) according to the manufacturer’s protocols for RNA clean-up. Final RNA preparations were resuspended in RNase-free water and stored at –80°C. The RNA samples were quantified spectrophotometrically, and purity and integrity were assessed by agarose gel electrophoresis. All samples exhib-ited 260/280 absorbance ratios of approximately 2.0, and all showed intact ribosomal 28S and 18S RNA bands in an approximate ratio of 2:1 as visu-alized by ethidium bromide staining.
Label
biotin
Label protocol
standard Affymetrix procedures
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures using GeneChip® Scanner 3000.
Description
a time series of ConA injections was administered and 20 mg/kg ConA was injected through the mouse caudal vein at one of 4 time points (0 hr, 1 hr, 3 hr, 6 hr),here is hepatic gene expression at "3 hr" (rep3)
