|
| Status |
Public on Jul 17, 2009 |
| Title |
NotSAT_BDntTS5 |
| Sample type |
genomic |
| |
|
| Source name |
TS cells derived from somatic-cell nuclear-transferred blastocyst
|
| Organism |
Mus musculus |
| Characteristics |
cell type: TS cells
source: somatic-cell nuclear-transferred blastocyst
|
| Treatment protocol |
Day 4 in proliferation medium.
|
| Growth protocol |
Cultured in proliferation medium for TS cells (RPMI1640 + 20%FBS + 1 mM Sodium Pyruvate + 2 mM L-glutamine + penicillin/storeptmycin + 25ng/ml bFGF + heparin)(Tanaka et al. Science (1988) 282:2072-2075)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was purified using proteinase K, Rnase A and phenol/chroloform, and amplified HpyCH4IV-TaqI fragment using the combination of restriction digestion and linker-mediated PCR (Yagi et al. Genome Res (2008) 18:1969-1978).
|
| Label |
biotin
|
| Label protocol |
Affymetrix Chromatin-Immunoprecipitation Assay Protocol
|
| |
|
| Hybridization protocol |
Affymetrix Chromatin-Immunoprecipitation Assay Protocol
|
| Scan protocol |
Affymetrix Chromatin-Immunoprecipitation Assay Protocol
|
| Description |
data definition: Normalized median log signal intensity
|
| Data processing |
median signal intensity of probe corresponding to a HpyCH4IV-TaqI fragment was defined as the fragment value (= VALUE in main data file)
|
| |
|
| Submission date |
Jul 15, 2009 |
| Last update date |
Jul 16, 2009 |
| Contact name |
Mayumi Oda |
| E-mail(s) |
[email protected]
|
| Organization name |
Keio University
|
| Street address |
35 Shinanomachi, Shinjuku
|
| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8857 |
| Series (2) |
| GSE17104 |
DNA methylation comparison of ntTS, TS and ES cells |
| GSE17158 |
Expression and DNA methylation profiling of ntTS and TS cells |
|
