|
| Status |
Public on Oct 31, 2009 |
| Title |
35S::HRE1 Hypoxic Replicate No. 2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
growth stage: seedling
ecotype: Columbia 0, 35S::HRE1
age: 7 days
germination: aerobic
growth conditions: Murashige-Skoog growth conditions, half-strength, sucrose 30 mM, 23°C, light/dark 12/12 photoperiod
treatment: hypoxic treatment
|
| Biomaterial provider |
PlantLab, Scuola Superiore Sant'Anna, Pisa, Italy
|
| Treatment protocol |
This sample represents hypoxic 35S::HRE1 Col-0. Arabidopsis seedlings, 7d-old.
|
| Growth protocol |
Arabidopsis thaliana, Columbia-0 ecotype, was used in all experiments. Seeds were sterilized with ethanol 70% (1 min), 0.85% sodium hypochlorite (10 min) and rinsed with sterile water (6 times). Seedlings were grown on Murashige and Skoog half-strength medium (agar 0.8%) containing 30 mM sucrose. The plates were incubated in the dark at 4°C for two days (vernalization, to promote homogeneous germination) and then transferred to 23°C for seven days under light/dark 12/12 photoperiod before the treatments. Seedlings were grown under aerobic conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from whole 7-day old plants using the RNeasy kit (Qiagen). RNA quality was assessed by agarose gel electrophoresis and spectrophotometry. RNA was processed for use on Affymetrix Arabidopsis Genome GeneChip arrays.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard hybridization protocol
|
| Scan protocol |
Affymetrix standard scan protocol
|
| Description |
no additional description required
|
| Data processing |
GC-RMA algorithm
|
| |
|
| Submission date |
Jul 15, 2009 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Pierdomenico Perata |
| E-mail(s) |
[email protected]
|
| Phone |
+39050881901
|
| Organization name |
Sant'Anna School of Advanced Studies
|
| Department |
Institute of Life Sciences
|
| Lab |
PlantLab
|
| Street address |
Via Guidiccioni 8-10
|
| City |
San Giuliano Terme |
| State/province |
Pisa |
| ZIP/Postal code |
56010 |
| Country |
Italy |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE17099 |
Effect of AtHRE1 and AtHRE2 overexpression in normoxia and hypoxia |
|
| Relations |
| Reanalyzed by |
GSE118579 |
