|
| Status |
Public on Jul 10, 2009 |
| Title |
06-14-05_Miller_U133Plus2_0_A9P4d07EST |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ cell in EST condition on day 7
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD34+ hematopoietic progenitor cells
developmental stage: proerythroblast
tissue type: primary cells from donated blood
|
| Treatment protocol |
EST [Erythropoietin (EPO) =4U/ml, Stem Cell Factor (SCF)=50ng/ml, and Transforming growth factor beta-1 (TGFbeta-1)=1.25ng/ml]
|
| Growth protocol |
After obtaining informed consent, human CD34+ cells were isolated in high purity from the peripheral blood of normal human volunteers. The cells were cultured at a concentration of 104 -105 cells/mL in medium supplemented with 4 U/mL EPO (Amgen, Thousand Oaks, CA), 50ng/ml SCF, and 1.25ng/ml TGFbeta1 (R&D systems) as described previously (PMID: 15367428). Cells were enumerated using an electronic cell counter (Coulter, Hialeah, FL). Adult and fetal hemoglobin was analysed by HPLC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells harvested and homogenate prepared on 10.20.04 -11.10.04. Stored at -80C. RNA prepared between Dec, 2004 - Jan, 2005. RNA was extracted using QIAshredder and Rneasy Minikit.
|
| Label |
biotin
|
| Label protocol |
standard affymetrix protocol
|
| |
|
| Hybridization protocol |
standard affymetrix protocol
|
| Scan protocol |
standard affymetrix protocol
|
| Description |
Pool of 3 samples:
Donor: CD052804/CD013004/CD050903
Age: 23/42/22
Sex: M/F/F
Day: d7/d7/d7
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Jul 10, 2009 |
| Last update date |
Jul 10, 2009 |
| Contact name |
Jeffery Lynn Miller |
| E-mail(s) |
[email protected]
|
| Phone |
301-480-1908
|
| Fax |
301-435-5148
|
| Organization name |
NIH/NIDDK
|
| Department |
Molecular Medicine Branch
|
| Lab |
Molecular Biology and Genetics Section
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7874 |
Effects of EPO and EST on erythroid maturation |
|
