|
| Status |
Public on Jul 14, 2009 |
| Title |
Majeti_NBM_HSC_004 |
| Sample type |
RNA |
| |
|
| Source name |
Normal human bone marrow hematopoietic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Normal human bone marrow hematopoietic stem cells
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent containing either glycogen or linear acrylamide according to the manufacturer's protocol, and then treated with DNaseI (Ambion).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix IVT labeling protocol. See Affymetrix Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, ten micrograms of adjusted cRNA fromeach sample was hybridized for 16 hours at 45 deg C to Affymetrix GeneChip
|
| Scan protocol |
Scanning was performed using the Affymetrix GeneChip 3000 Scanner. Images were processed into CEL files with the Affymetrix GCOS software.
|
| Description |
Lin-CD34+CD38-CD90+
|
| Data processing |
Raw CEL intensity data were GCRMA normalized using R/Bioconductor
|
| |
|
| Submission date |
Jul 09, 2009 |
| Last update date |
Jul 13, 2009 |
| Contact name |
Qiang Tian |
| E-mail(s) |
[email protected]
|
| Phone |
206-732-1308
|
| Fax |
206-732-1299
|
| URL |
http://www.systemsbiology.org
|
| Organization name |
Institute for Systems Biology
|
| Lab |
Hood
|
| Street address |
1441 N 34th St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE17054 |
Dysregulated gene expression networks in human acute myelogenous leukemia stem cells |
|
