|
| Status |
Public on Jul 07, 2009 |
| Title |
OE-es-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis OE in iron plus medium
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Col-0
genome/variation: CsUBC13 overexpression
tissue: root
|
| Growth protocol |
10-day old seedlings were transferred to ES (+Fe) and ES without addition of FeEDTA (–Fe) medium, respectively. Tissues were harvested and immediately frozen in liquid nitrogen 3 days after the transfer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy mini kit.
|
| Label |
Biotin
|
| Label protocol |
Ten microgram of total RNA was used for cDNA synthesis, labeled by in vitro transcription, followed by fragmentation according to the manufacturer's suggestion (GeneChip Expression Analysis Technical Manual rev5, Affymetrix).
|
| |
|
| Hybridization protocol |
Ten microgram labeled samples were hybridized to the ATH1 Genome Array at 45℃ for 16.5 h. Washing and staining involved Fluidics Station-450 and the ATH1 Genome.
|
| Scan protocol |
Array was scanned with the Affymetrix GeneChip Scanner 7G.
|
| Description |
gene expression data from transgenic arabidopsis roots under iron efficiency medium
|
| Data processing |
The results were quantified and analyzed by use of MicroArray Suite 5.0 software (Affymetrix Inc.)
|
| |
|
| Submission date |
Jul 06, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Wolfgang Schmidt |
| E-mail(s) |
[email protected]
|
| Phone |
+886-2-2787-1162
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Plant and Microbial Biology
|
| Street address |
No. 128, Sec 2, Academia Rd, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE16964 |
Iron-deficiency-induced changes in wild type, ubc13A and cucumber CsUBC13 overexpressed Arabidopsis roots |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
