|
| Status |
Public on Dec 01, 2021 |
| Title |
RLN (17-15) |
| Sample type |
RNA |
| |
|
| Source name |
Non-neoplastic Reactive Lymph Node (RLN)
|
| Organism |
Homo sapiens |
| Characteristics |
sox11 status: -
tissue: Lymph node
treatment: -
tumoral content: -
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Nucleic acids were extracted from 10-µm sections of FFPE biopsies from lymph nodes using the QIAGEN AllPrep DNA/RNA FFPE kit (QIAGEN, Hilden, Germany) after deparaffinization according to the manufacturer´s instructions.
|
| Label |
n/a
|
| Label protocol |
Samples were labeled with Nanostring Reporter Probes.
|
| |
|
| Hybridization protocol |
Hybridization master mix was prepared and split into the 12 tubes containing the samples to analyze. Samples were then incubated in a PCR machine, for 16hours at 67ºC.
|
| Scan protocol |
cartridges were scanned and counted using the Nanostring scanner at its higher resolution (555 FOVs).
|
| Data processing |
Normalitzation with nSolver Analysis Software 4.0. Standard normalization uses a combination of Positive Control Normalization, which uses synthetic positive control targets, and CodeSet Content Normalization, which uses housekeeping genes, to apply a sample-specific correction factor to all the target probes within that sample lane.
|
| |
|
| Submission date |
Dec 05, 2019 |
| Last update date |
Dec 01, 2021 |
| Contact name |
Virginia Amador |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
932275400
|
| Organization name |
IDIBAPS
|
| Lab |
Functional characterization of oncogenic mechanisms in lymphomagenesis
|
| Street address |
Roselló 149-153
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL25262 |
| Series (1) |
| GSE141539 |
Expression profiles of SOX11-positive and SOX11-negative Mantle Cell Lymphoma (MCL) primary samples from lymph node and reactive lymph nodes (RLN). |
|
