|
| Status |
Public on Dec 16, 2011 |
| Title |
DU145N 24h noHGF |
| Sample type |
RNA |
| |
|
| Source name |
DU145 cells 24 hours with vehicle
|
| Organism |
Homo sapiens |
| Characteristics |
time point: 24 hours
agent: vehicle
cell line: In vitro prostate cancer DU145 cell line
|
| Treatment protocol |
One day after medium replacement, DU145 prostate cancer cells were treated with 25 ng/ml hepatocyte growth factor (HGF) or vehicle for 2, 8, or 24 hours.
|
| Growth protocol |
DU145 were seeded overnight in RPMI/ FCS medium. After 1 day, medium was replaced by RPMI medium containing 5% Dextran Charcoal treated (DCC).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNAzol B reagent. After standard RNA isolation with chloroform, isopropanol and ethanol, DNA was digested using DNA-free kit. RNA quality and quantity was measured with the RNA 6000 Nano kit on a 2100 Bioanalyzer. Samples with RNA integrity numbers of >8.5 were selected.
|
| Label |
biotin
|
| Label protocol |
5 μg of total RNA from stimulated and control samples was used to prepare antisense biotinylated RNA according to manufacturer’s one-cycle protocol (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Hybridization to Affymetrix Human U133plus2.0 GeneChips (54,614 probe sets, representing approximately 47,000 transcripts), staining, washing, and scanning procedures were performed as described by Affymetrix (Affymetrix, Santa Clara, CA, USA), and performed by Erasmus MC Center for Biomics.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner.
|
| Description |
expression data of HGF/cMET pathway in prostate cancer DU145 cell line
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 17, 2009 |
| Last update date |
Dec 17, 2011 |
| Contact name |
Guido Jenster |
| E-mail(s) |
[email protected]
|
| Phone |
31107043672
|
| Organization name |
Erasmus MC
|
| Department |
Urology
|
| Lab |
JNI, Be362a
|
| Street address |
P.O. Box 2040
|
| City |
Rotterdam |
| ZIP/Postal code |
3000CA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE16659 |
Expression data of HGF/cMET pathway in prostate cancer DU145 cell line |
|
