|
| Status |
Public on Nov 01, 2022 |
| Title |
18_KO (F480low CD11b High Macrophages ) |
| Sample type |
SRA |
| |
|
| Source name |
Kidney
|
| Organism |
Mus musculus |
| Characteristics |
genotype: KO
|
| Treatment protocol |
Cells were stimulated with 100 ng/ml LPS in complete RPMI for 2hrs at 37C with 5% CO2 after which cells were sorted as F4/80 low CD11b high
|
| Growth protocol |
Cell suspensions were prepaired fresh from murine kidneys
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were homoginised in RLT+ (Qiagen) and RNA extracted with Rneasy plus micro kit (Qiagen)
Libraries were constructed with Takara pico input stranded
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
18_KO
|
| Data processing |
Demultiplex with Casava
Alignment with Hisat2
Genome_build: Mm10
|
| |
|
| Submission date |
Nov 01, 2019 |
| Last update date |
Nov 01, 2022 |
| Contact name |
John Robert Ferdinand |
| Organization name |
University of Cambridge
|
| Street address |
Department of Medicine
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE139854 |
Analysis of LPS stimulation on F480low CD11b High Macrophages from WT and muMT KO mice |
|
| Relations |
| BioSample |
SAMN13181531 |
| SRA |
SRX7087569 |
