|
| Status |
Public on Jul 31, 2010 |
| Title |
Gastrocnemius Muscle_Cmah KO_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrocnemius, Cmah KO, 2 months old
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype: Cmah KO
gender: male
age: 2 months
tissue: Gastrocnemius muscle
|
| Growth protocol |
All mice were maintained in the institute animal facility following the IACUC guidelines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions followed by further purification on a silica-gel-based membrane (RNeasy-Mini; Qiagen, Valencia, CA).
|
| Label |
Biotin
|
| Label protocol |
The Ovation™ Biotin RNA Amplification and Labeling System (NuGen Technologies, Inc., San Carlos, CA) was used to prepare amplified, biotin-labeled cDNA from 25 ng of total RNA following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
For each array, 2.2 µg of fragmented and labeled cDNA was hybridized onto 430 2.0 GeneChips® (Affymetrix Inc., USA), for 16 hours at 45ºC followed by washing and staining of microarrays in a Fluidics Station 450 (Affymetrix Inc., USA).
|
| Scan protocol |
GeneChips were scanned in a GeneChip Scanner 3000 (Affymetrix Inc., USA).
|
| Description |
Gene expression data from skeletal muscle of two-month-old mouse deficient in Neu5Gc sialic acid.
5Cmah KO Skeletal Muscle
|
| Data processing |
CEL files were generated from DAT files using GeneChip® Operating Software (GCOS) software (Affymetrix Inc., USA) and probe set signals were generated using the RMA algorithm in ArrayAssist 3.4 (Stratagene). Differential gene expression was determined by pair-wise comparisons.
|
| |
|
| Submission date |
Jun 04, 2009 |
| Last update date |
Dec 21, 2009 |
| Contact name |
Paul T Martin |
| E-mail(s) |
[email protected]
|
| Organization name |
Columbus Children's Research Institute
|
| Street address |
700 Children's Drive
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE16438 |
Array profiling of dystrophin-deficient mice with a secondary glycosylation defect |
|
