|
| Status |
Public on Jun 03, 2009 |
| Title |
HB00GAH |
| Sample type |
RNA |
| |
|
| Source name |
Omentum (visceral adipose ) tissue from diabetic subject
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: type-2 diabetes mellitus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Adipose tissue was collected in RNA Later. Total RNA was extracted using Ribopure kit from Ambion.
|
| Label |
digoxigenin (DIG)
|
| Label protocol |
Followed one round (standard) of amplification. NanoAmp RT-IVT Labeling kit from Applied Biosystems was used to convert RNA into digoxigenin (DIG)-labeled cRNA for hybridisation to Applied Boisystems microarrays
|
| |
|
| Hybridization protocol |
Applied Biosystems Chemiluminescence Detection kit was used for hybridising the cRNA to Applied Boisystems 1700 Human genome survey microarrays
|
| Scan protocol |
The AB 1700 Human Genome survey microarrays were scanned using Applied Biosystems 1700 Chemiluminescent Analyzer following the manufacturer's protocol
|
| Description |
no additional information
|
| Data processing |
The data was initially analysed by AB array which is a software package written in R for the quality assessment and automated statistical analysis of Applied Boisystems expression array system data. AB array provides Quality control graphical output, data integrity graphical output, statistical processing and statistical graphical output.The data is then analysed using spotfire statistical software. The data is first normalised (trim mean normalization) and samples are being grouped into controls and samples. The two groups are then compared for fold change expression and the data is then merged with the pathways wrt probe IDs and Gene IDs.
|
| |
|
| Submission date |
Jun 03, 2009 |
| Last update date |
Jun 03, 2009 |
| Contact name |
Sandeep Kumar Mathur |
| E-mail(s) |
[email protected]
|
| Phone |
+9194414048666
|
| Organization name |
S.M.S.Medical College
|
| Department |
Endocrinology
|
| Street address |
JLN Marg
|
| City |
Jaipur |
| State/province |
Rajasthan |
| ZIP/Postal code |
302015 |
| Country |
India |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE16415 |
Genome wide gene expression profiling of visceral adipose tissue among Asian Indian diabetics |
|
