|
| Status |
Public on Jun 08, 2009 |
| Title |
HeLa cells treated for 6 h |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
agent: MT7
time: 6 h
|
| Treatment protocol |
HeLa cells treated with 5 mM MT7 for 0(control), 1.5(Time1), 3(Time2), 6(Time3) and 12(Time4) hours, respectively, were then washed three times with PBS and the total RNA was isolated and processed for Affymetrix U133A 2.0 hybridizations.
|
| Growth protocol |
HeLa cells were grown in RPMI1640 plus 10% fetal bovine serum as the supplier's instruction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using QIAGEN RNeasy Mini Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Gene expression data from embryos in slow phase of cellularisation.
|
| Data processing |
The data were analyzed with GCOS software
|
| |
|
| Submission date |
Jun 02, 2009 |
| Last update date |
Jun 07, 2009 |
| Contact name |
Zhixiang Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
SIMM
|
| Street address |
Rd. Zuchongzhi
|
| City |
Shanghai |
| ZIP/Postal code |
201203 |
| Country |
China |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE16378 |
The kinetics of gene expressions of HeLa cells treated with MT7 |
|
