|
| Status |
Public on Nov 20, 2009 |
| Title |
siRNA, plastic plate, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
siRNA-3-PLASTIC
|
| Organism |
Homo sapiens |
| Characteristics |
sirna: Syk siRNA knockdown
plate: plastic
cell line: MCF10A
|
| Treatment protocol |
Syk siRNA knockdown or control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
per Affymetrix Genechip Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
siRNA, plastic plate
|
| Data processing |
Raw images were processed using Microarray Suite 5.0 software. For the processing of the microarray data, we used SemiRMA (Bebu et al., 2007).
|
| |
|
| Submission date |
May 22, 2009 |
| Last update date |
Nov 20, 2009 |
| Contact name |
Junfeng Sun |
| Organization name |
Georgetown Univ
|
| Street address |
Suite 180, 4000 Reservior Rd NW
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20057 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE16200 |
Loss of Syk in normal breast cells |
|
