|
| Status |
Public on May 18, 2010 |
| Title |
At_without DEX_T0 |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis leaf without DEX treatment for 0 hr.
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: Young rosette leaves (5-7th)
genotype: Columbia (Col-0)
age: 14-days old soil grown seedling
|
| Treatment protocol |
DEX (dexamethasone, Sigma) treatment was performed to the transgenic Arabidopsis (inducible expression of MYB46) plants were grown on soil for 14 days. DEX was dissolved in water to 1 mM stock and applied with spray at 10 µM with 0.02% silwet surfactant. In relevant experiments, control plants were treated with the same concentration of surfactant in water. After treatment, young rosette leaves (5th ~7th leaf) without petiole were harvested at the indicated times and frozen immediately with liquid N2, then stored until used.
|
| Growth protocol |
The transgenic Arabidopsis (inducible expression of MYB46) plants were grown on soil in a growth chamber (16 h light/ 8 h dark) at 23 °C, after stratification.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from Arabidopsis leaf without DEX treatment for 0 hr.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 18, 2009 |
| Last update date |
May 18, 2009 |
| Contact name |
Jae-Heung Ko |
| E-mail(s) |
[email protected]
|
| Phone |
82-31-201-3863
|
| Organization name |
Kyung Hee University
|
| Department |
Plant & Environmental Resources
|
| Street address |
1 Seocheon-dong, Giheung-gu
|
| City |
Yongin |
| State/province |
Gyeonggi |
| ZIP/Postal code |
446-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE16143 |
Ectopic expression of MYB46 identifies transcriptional regulatory genes in secondary wall biosynthesis in Arabidopsis |
|
