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Sample GSM403932 Query DataSets for GSM403932
Status Public on Jun 03, 2009
Title SLN:1894 - A - mAdbID:39699
Sample type RNA
 
Source name SLN:1894
Organism Homo sapiens
Characteristics tissue: Lymph node
disease state: Follicular Lymphoma
individual: SLN:1894
clinical info: Follow up status: dead
clinical info: Follow up years: 11.37
clinical info: Stage >2: yes
clinical info: LDH ratio >1: no
clinical info: Number of extranodal sites >1: yes
clinical info: Age >60: no
clinical info: IPI score: 2
clinical info: Immune response 1: 9.01
clinical info: Immune response 2: 8.64
clinical info: With translocation: yes
Extracted molecule polyA RNA
Extraction protocol FastTrack 2.0 mRNA Isolation Kit
Extraction method: oligo dt cellulose
Other: mRNA was extracted using the FastTrack 2.0 mRNA Isolation Kit according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
Label biotin
Label protocol Affymetrix Labeling Protocol
Other: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Affymetrix Hybridization Protocol
Other: Following fragmentation, 20 micrograms of cRNA were hybridized for 16 hours at 45C on U133A/B GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Affy Scanning Protocol
Other: Scanning was performed by the Affymetrix 3000 Scanner.
Description mAdb experiment ID: 39699
Data processing MAS 5.0 Data Processing Protocol
Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MA S 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date May 15, 2009
Last update date Jun 03, 2009
Contact name Louis M. Staudt
E-mail(s) [email protected]
Phone 301-402-1892
Organization name National Cancer Institute
Department Lymphoid Malignancies Branch
Lab Louis M Staudt
Street address 9000 Rockville Pike, Bldg 10, Rm 4N114
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL96
Series (1)
GSE16131 Differences Between Follicular Lymphoma With and Without Translocation t(14;18)

Data table header descriptions
ID_REF Affymetrix ID
VALUE log2 of MAS5-calculated Signal intensity

Data table
ID_REF VALUE
117_at 7.823
121_at 10.078
177_at 6.318
179_at 9.786
320_at 8.475
336_at 7.577
564_at 8.102
632_at 9.561
823_at 7.439
1053_at 7.198
1294_at 10.608
1316_at 6.837
1320_at 4.517
1431_at 6.746
1438_at 6.127
1487_at 9.455
1729_at 9.877
1773_at 7.009
1861_at 7.994
243_g_at 9.755

Total number of rows: 22283

Table truncated, full table size 372 Kbytes.




Supplementary file Size Download File type/resource
GSM403932.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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