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Sample GSM402683 Query DataSets for GSM402683
Status Public on Sep 08, 2009
Title Saos-2 resistant cells replicate 3
Sample type RNA
 
Source name Saos-2 methotrexate-resistant cells
Organism Homo sapiens
Characteristics cell line: Saos-2
cell type: osteosarcoma
methotrexate sensitivity: resistant
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit from Qiagen
Label biotin
Label protocol Affymetrix protocol for HG U133 PLUS 2.0
 
Hybridization protocol Affymetrix protocol for HG U133 PLUS 2.0
Scan protocol Affymetrix protocol for HG U133 PLUS 2.0
Description Gene expression profile of Saos-2 cells resistant to 10e-6M methotrexate.
Data processing Value generated using MAS 5.0 non-scaled
 
Submission date May 13, 2009
Last update date Jun 18, 2009
Contact name Carlos J Ciudad
E-mail(s) [email protected]
Phone +34-93-403-4455
Organization name University of Barcelona
Department Biochemistry and Molecular Biology
Lab School of Pharmacy
Street address Av. Juan XXIII-27
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
 
Platform ID GPL570
Series (2)
GSE16089 Networking of differentially expressed genes in human Saos-2 osteosarcoma cells resistant to methotrexate
GSE16648 Networking of differentially expressed genes in human cancer cell lines resistant to methotrexate

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal
ABS_CALL Present or absent
Detection p-value Detection p-value

Data table
ID_REF VALUE ABS_CALL Detection p-value
AFFX-BioB-5_at 175.4 P 0.000258
AFFX-BioB-M_at 266.4 P 0.000044
AFFX-BioB-3_at 151 P 0.000044
AFFX-BioC-5_at 551.3 P 0.000052
AFFX-BioC-3_at 623.4 P 0.000044
AFFX-BioDn-5_at 1365.6 P 0.000044
AFFX-BioDn-3_at 2872.5 P 0.000044
AFFX-CreX-5_at 6013 P 0.000052
AFFX-CreX-3_at 7341.2 P 0.000044
AFFX-DapX-5_at 0.9 A 0.724871
AFFX-DapX-M_at 9.9 P 0.028457
AFFX-DapX-3_at 1.2 A 0.891021
AFFX-LysX-5_at 1.9 A 0.51489
AFFX-LysX-M_at 1.5 A 0.712257
AFFX-LysX-3_at 6.8 A 0.089478
AFFX-PheX-5_at 0.9 A 0.824693
AFFX-PheX-M_at 0.9 A 0.904333
AFFX-PheX-3_at 1.8 A 0.760937
AFFX-ThrX-5_at 3.3 A 0.672921
AFFX-ThrX-M_at 4.6 A 0.631562

Total number of rows: 54675

Table truncated, full table size 1399 Kbytes.




Supplementary file Size Download File type/resource
GSM402683.CEL.gz 7.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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