|
| Status |
Public on May 28, 2009 |
| Title |
UPN12_T-cell |
| Sample type |
genomic |
| |
|
| Source name |
T-cell fraction
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: T-cell fraction
disease state: MDS
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
QiaAmp purification kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated DNA was prepared according to the standard Affymetrix protocol from 250 ng genomic DNA (Genechip Mapping 500k assay manual 701930 Rev.3 or 100k assay manual 701684 Rev.3, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridizations were performed according to the standard Affymetrix protocol from 250 ng genomic DNA (Genechip Mapping 500k assay manual 701930 Rev.3 or 100k assay manual 701684 Rev.3, Affymetrix) using an Affymetrix hybridisation oven 640 and an Affymetrix Fluidic station 450.
|
| Scan protocol |
Scanning performed according to the standard Affymetrix protocol from 250 ng genomic DNA (Genechip Mapping 500k assay manual 701930 Rev.3 or 100k assay manual 701684 Rev.3, Affymetrix) using an Affymetrix scanner 3000.
|
| Description |
FACs-sorted T-cell fraction
|
| Data processing |
CNAG 2.0 and Affymetrix Genotypic Console 2.1
|
| |
|
| Submission date |
Apr 15, 2009 |
| Last update date |
May 28, 2009 |
| Contact name |
Roland P. Kuiper |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
+31243610868
|
| Fax |
+31243668752
|
| Organization name |
Radboud University Nijmegen Medical Centre
|
| Department |
Human Genetics
|
| Street address |
Geert Grooteplein 10
|
| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL3718 |
| Series (1) |
| GSE15688 |
Acquired mutations in TET2 are common in myelodysplastic syndromes |
|
