|
| Status |
Public on Jun 01, 2009 |
| Title |
Liver_Lean_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 25
body mass index (kg/m2): 25.5
hemoglobin a1c: 5.0
fasting glucose: 94.5
fasting insulin: 14.8
si: na
sg: na
cholesterol: na
triglycerides: na
hdl: na
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15ug of biotinylated cRNA were hybridized for 16hr at 45C to the Affymetrix GeneChip Human Genome U133A array. Following hybridization, arrays were washed and stained on the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner G2500.
|
| Description |
Gene expression from liver of lean subject
|
| Data processing |
Data were analyzed using Affymetrix GCOS v.1.3, MAS5.0 algorithm, and globally scaled with the trimmed mean target intensity set to 1500.
|
| |
|
| Submission date |
Apr 13, 2009 |
| Last update date |
Apr 13, 2009 |
| Contact name |
Mary Elizabeth Patti |
| E-mail(s) |
[email protected]
|
| Phone |
6177351966
|
| Fax |
6177322593
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Research Division
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE15653 |
Expression data from liver of obese (with or without type 2 diabetes) and lean human subjects. |
|
