|
| Status |
Public on Jun 18, 2019 |
| Title |
isolated cDC2s pSS_08 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
age: 50
|
| Treatment protocol |
Peripheral blood CD1c-expressing cDC2s were isolated using magnetic-activated cell sorting using CD1c (BDCA-1)+ Dendritic Cell Isolation Kit (Miltenyi Biotec)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen)
|
| Label |
TaqMan
|
| Label protocol |
Samples were prepared using theTaqman OpenArray Human microRNA protoco,l panel version miRBase 20.1 (Life Technologies catalog number 4470187)
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
isolated cDC2s
primary Sjögren's Syndrome patient
pSS_08
|
| Data processing |
miRNA profiling data was analyzed using the ExpressionSuite Software (Life Technologies) according to the comparative threshold cycle (CT) method.
Low expressed miRNAs (CT > 27) were set at 27.
The expression of each sample was normalized by the mean expression of the four reference sncRNAs (miR-17, miR-191, RNU48, U6-snRNA).
The miRNA expression normalized to the reference sncRNAs was calculated using 2^-deltaCt, where deltaCt = (Ct_miRNA − meanCt_sncRNAs)
Relative expression was depicted as FC compared to one randomly selected healthy control (HC_03), which was set at 1.
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Non-normalized data reports raw CT value.
Fold Change data reports the relative expression of each sample compared to a randomly selected healthy control sample (HC_03) for the 143 expressed miRNAs.
|
| |
|
| Submission date |
Jun 17, 2019 |
| Last update date |
Jun 18, 2019 |
| Contact name |
Ana Pinheiro Lopes |
| E-mail(s) |
[email protected]
|
| Organization name |
University Medical Center Utrecht
|
| Street address |
Heidelberglaan 100
|
| City |
Utrecht |
| State/province |
Utrecht |
| ZIP/Postal code |
3584 CX |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL22992 |
| Series (1) |
| GSE132842 |
MicroRNA-130a Contributes to Type-2 Classical DC-activation in Sjögren’s Syndrome by Targeting MSK-1 |
|
