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Sample GSM3891

Query DataSets for GSM3891

Status

Public on Feb 13, 2003

Title

HU10Kb480

Sample type

RNA

Channel 1

Source name

Stratagene universal reference

Organism

Extracted molecule

total RNA

Channel 2

Source name

Embryonic lung fibroblasts (HLFs)

Organism

Extracted molecule

total RNA

Description

HLFs were infected with lentiviruses pAB319 and pAB322 expressing MRG15 and MRGX small hairpin RNAs from the H1 polIII promoter. RNA was amplified by one round of T7 transcription and labelled by reverse transcription in the presence of Cy3 and Cy5 dCTP. The pAB319 shRNA induces an artefactual interferon response.

Submission date

Feb 11, 2003

Last update date

Nov 21, 2005

Contact name

Richard Iggo

E-mail(s)

[email protected]

Phone

+33 5 56 33 04 23

Fax

+33 5 56 33 32 06

Organization name

University of Bordeaux

Department

Bergonie Cancer Institute

Lab

INSERM U916

Street address

229 cours de l'Argonne

City

Bordeaux

ZIP/Postal code

33076

Country

France

Platform ID

Series (1)

Interferon induction by shRNA

Data table header descriptions
ID_REF
VALUE	same as UNF_VALUE but with flagged values removed
CH1I	Scanalyze Ch1 intensity
CH1B	Scanalyze Ch1 background
CH2I	Scanalyze Ch2 intensity
CH2B	Scanalyze Ch2 background
RAT2	Scanalyze Ratio of mean intensities
MRAT	Scanalyze Median ratio of intensities
FLAG	Manual quality flag (1 = bad)
UNF_VALUE	Log2 ratio, no bkg subtraction, S normalisation in sma in R

Data table
ID_REF	VALUE	CH1I	CH1B	CH2I	CH2B	RAT2	MRAT	FLAG	UNF_VALUE
1		14608	158	15556	95	1.07	0.9947	0
2	0.7671	2267	141	2854	90	1.3	1.147	0	0.7671
3	1.5574	3832	131	15413	95	4.139	3.807	0	1.5574
4	-0.2812	625	130	373	93	0.5657	0.5287	0	-0.2812
5	-0.5998	1363	125	583	87	0.4006	0.4177	0	-0.5998
6	1.2863	3649	125	11490	89	3.235	3.109	0	1.2863
7	3.2209	1429	126	12459	86	9.496	7.39	0	3.2209
8	0.5868	389	131	396	83	1.213	1.034	0	0.5868
9	0.8503	2096	138	2732	84	1.352	1.312	0	0.8503
10	-0.4812	5235	143	4529	88	0.8722	0.8792	0	-0.4812
11	0.0935	5157	147	7327	85	1.446	1.233	0	0.0935
12		1143	152	639	80	0.5641	0.5972	0
13	0.8871	545	140	691	83	1.501	1.367	0	0.8871
14	0.0214	7997	140	13059	90	1.651	1.58	0	0.0214
15	0.8137	2105	137	2670	89	1.311	1.195	0	0.8137
16	1.6078	870	150	1506	89	1.968	1.394	0	1.6078
17	-0.3381	3035	150	1809	85	0.5976	0.5877	0	-0.3381
18	0.5001	2350	149	2438	89	1.067	1.006	0	0.5001
19	-0.0835	4409	178	4691	96	1.086	1.016	0	-0.0835
20	0.6787	2913	166	4104	94	1.46	1.118	0	0.6787

Total number of rows: 10368

Table truncated, full table size 502 Kbytes.

Supplementary data files not provided

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