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Sample GSM388135 Query DataSets for GSM388135
Status Public on Jun 13, 2009
Title T40875
Sample type RNA
 
Source name pancreas
Organism Homo sapiens
Characteristics patient: 40875
sample: tumor
Treatment protocol Pairs of normal and tumor tissue samples were obtained at the time of surgery from resected pancreas of 36 pancreatic cancer patients. The tissue samples were snap-frozen in liquid nitrogen immediately upon surgical removal and maintained at −85°C. For histological evaluation, sections were stained with haematoxylin and eosin. The resected cancer specimens were not microdissected.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the frozen tissue samples in two steps: RNA isolation using the TriReagent (Sigma) protocol, followed by RNA clean-up performed as described in the RNeasy (Qiagen) protocol. Briefly, tissues were homogenized in TriReagent (1 mL of TriReagent per 50-100mg of tissue). Then, RNA was precipitated from the aqueous phase with addition of isopropanol at half the volume of the original TriReagent volume and centrifugated at 12000 × g for 10 min. RNA was cleaned using an RNeasy column according to the manufacturer’s instructions (RNeasy Mini Kit, Qiagen) and eluted in 100 µL of RNase-free water. RNA sample quantitation was performed by means of spectrophotometry (Genequant, Pharmacia), while RNA quality (integrity) control was done with microfluidics technology (2100 Bioanalyser, Agilent Technologies).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol The fragmented cRNA was hybridized to the oligonucleotide microarray, which was washed and stained with streptavidin-phycoerythrin.
Scan protocol Scanning was performed with an Affymetrix Microarray Scanner.
Description Gene expression data from surgical samples
Data processing Affymetrix GeneChip Operating Software (GCOS) Version 1.4 was used for low-level scanning data processing. The data was subsequently normalized using the RMA algorithm using the Bioconductor 1.9 package (http://www.bioconductor.org/) running under R version 1.9.1.
 
Submission date Mar 31, 2009
Last update date Aug 28, 2018
Contact name Liviu Badea
E-mail(s) [email protected]
Organization name ICI
Department Research
Lab AI and Bioinformatics
Street address 8-10 Averescu Blvd.
City Bucharest
ZIP/Postal code 011455
Country Romania
 
Platform ID GPL570
Series (1)
GSE15471 Whole-Tissue Gene Expression Study of Pancreatic Ductal Adenocarcinoma
Relations
Reanalyzed by GSE51798
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2 values)

Data table
ID_REF VALUE
1007_s_at 10.14183816
1053_at 6.116951638
117_at 6.080201664
121_at 6.921877374
1255_g_at 2.902900072
1294_at 7.901457727
1316_at 5.183522768
1320_at 5.095717044
1405_i_at 7.25751869
1431_at 4.079406978
1438_at 5.51733641
1487_at 6.64378179
1494_f_at 4.618627546
1552256_a_at 7.348755126
1552257_a_at 8.311735248
1552258_at 4.803101356
1552261_at 4.9663937
1552263_at 5.513508853
1552264_a_at 6.946163042
1552266_at 3.912214521

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM388135.CEL.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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