|
| Status |
Public on Apr 01, 2009 |
| Title |
RAW_0mM_HOCl_#1 |
| Sample type |
RNA |
| |
|
| Source name |
RNA from RAW 264.7 Cells
|
| Organism |
Mus musculus |
| Characteristics |
Array Type; Affymetrix Mouse 430_2
amount: 5ug
bioanalyzer results: Good
cell type: RAW 264.7
|
| Biomaterial provider |
Courtney G. Woods, The Hamner Institutes, 6 Davis Dr, RTP, NC, 27709, [email protected] 919 558 1267, 919 558 1300
|
| Treatment protocol |
Treatment: 0 mM HOCl in medium
Medium: see growth protocol below
Treatment Duration: 6 hours
Cell Confluency upon Treatment : ~80-90%
|
| Growth protocol |
RAW 264.7 macrophages (RAW cells) were cultured in DMEM supplemented with 10% FBS, 10 mM HEPES, 100 U penicillin/ml, and 100 µg streptomycin/ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Triazol
|
| Label |
biotin
|
| Label protocol |
standard affymetrix protocol
|
| |
|
| Hybridization protocol |
standard affymetrix protocol
|
| Scan protocol |
standard affymetrix protocol
|
| Description |
no additional information
|
| Data processing |
Robust Multi-array Average
|
| |
|
| Submission date |
Mar 30, 2009 |
| Last update date |
Mar 31, 2009 |
| Contact name |
Courtney G Woods |
| E-mail(s) |
[email protected]
|
| Phone |
919-558-1267
|
| Fax |
919-558-1300
|
| Organization name |
The Hamner Institutes
|
| Department |
Computational Biology
|
| Street address |
PO Box 12137, 6 Davis Drive
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15457 |
Gene Expression Profiling of Cellular Dose-Response to Hypochlorous Acid (HOCl) |
|
