|
| Status |
Public on Mar 26, 2009 |
| Title |
sa6-fosf-1-20min |
| Sample type |
RNA |
| |
|
| Source name |
fosformycin 1ug 20min
|
| Organism |
Staphylococcus aureus |
| Characteristics |
strain: ATTC 29213
|
| Treatment protocol |
Bacterial cultures were treated with 0, 1 and 4 µg/ml, respectively, of fosfomycin. Bacterial cells were harvested at the start of the experiment (t0) and at 3 time points (10, 20 and 40 minutes) after treatment. The experiment was repeated at least three times.
|
| Growth protocol |
Staphylococcus aureus, strain ATTC 29213 was used in the experiments. Overnight culture was set in a small volume of cation-adjusted Mueller–Hinton broth medium (Sigma-Aldrich) and grown in Erlenmeyer flask on gyratory shaker (200 rpm) at 37°C. The overnight culture was diluted 100-fold in 300 ml of medium and grown under same conditions in 1-L Erlenmeyer flasks until OD600 (measured by Beckman DU640 spectrophotometer) reached 0.3, which corresponded to the early exponential stage of the growth curve.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from an aliquot of 7ml of the culture using enzyme cell wall lysis (30) followed by RNeasy Mini Kit (Qiagen) purification.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
sa6-fosf-1-20min 0028-6747.cel
|
| Data processing |
The data were analyzed with Expression Console version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 25, 2009 |
| Last update date |
Mar 25, 2009 |
| Contact name |
Drago Kuzman |
| E-mail(s) |
[email protected]
|
| Organization name |
Lek pharmaceuticals d.d.
|
| Street address |
Verovskova 57
|
| City |
Ljubljana |
| ZIP/Postal code |
SI-1000 |
| Country |
Slovenia |
| |
|
| Platform ID |
GPL1339 |
| Series (1) |
| GSE15394 |
Staphylococcus aureus treated with fosfomycin |
|
