|
| Status |
Public on Mar 26, 2009 |
| Title |
DU145 cells treated at the IC90 for 6 hours, replicate d |
| Sample type |
RNA |
| |
|
| Source name |
DU145 cells treated at the IC90 for 6 hours, replicate d
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
|
| Treatment protocol |
DU145 cells were grown in vehicle (DMSO), IC50, IC90 or the 3xIC90 dose of R547 for 0, 2, 6, and 24 hours
|
| Growth protocol |
Cell lines were maintained in the recommended medium and supplemented with 10% heat-inactivated fetal bovine serum (Life Technologies, Gaithersburg, MD) and 2 mmol/L L-glutamine (Life Technologies)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the Qiagen RNeasy Mini Kit
|
| Label |
biotin
|
| Label protocol |
RNA was converted into cDNA and cRNA according to the manufacturer’s recommendation and using manufacturer’s kits (Affymetrix, Santa Clara, CA). 15 ug of total RNA was converted to cDNA with the One-cycle cDNA Synthesis Kit with the addition of reagents from the Poly-A RNA Control Kit. cRNA was produced with the IVT Labeling Kit
|
| |
|
| Hybridization protocol |
Hybridization Mix contained, in addition to cRNA, reagents from the Hybridization Control Kit and Control Oligo B2. Staining and washing steps were performed as suggested by the manufacturer (Affymetrix, Santa Clara, CA).
|
| Scan protocol |
Each hybridized Affymetrix GeneChip array was scanned with a GeneChip Scanner 3000 7G (Agilent/Affymetrix).
|
| Description |
n/a
|
| Data processing |
Affymetrix gene expression microarray data was first background corrected and normalized via the RMA procedure followed by QQ normalization (R/Bioconductor)
|
| |
|
| Submission date |
Mar 25, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
James Rosinski |
| E-mail(s) |
[email protected]
|
| Organization name |
Roche
|
| Department |
In Silico Sciences
|
| Street address |
340 Kingsland St
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE15392 |
DU145 tumor cells treated with a CDK inhibitor |
| GSE15396 |
Peripheral blood mononuclear, DU145, and HCT116 cells treated with a CDK inhibitor |
|
| Relations |
| Reanalyzed by |
GSE119087 |
