|
| Status |
Public on Dec 24, 2019 |
| Title |
WT_37C_5min_r3 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast wild type cells kept 5min at 37°C
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
treatment: cells kept 5min at 37°C
strain: BY4147
genotype: MATa; his3D1; leu2D0; met15D0; ura3D0
batch: A863
|
| Treatment protocol |
cells were submitted to the corresponding thermal stress in a water bath with the corresponding temperature for the corresponding time span
|
| Growth protocol |
cells were grown in 50 ml YPD from an OD595 of 0.2 to an OD595 of 0.8
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were harvested (1min, 4000 x g, 4°C) and washed one time with 25ml ice cold H2O. The cells were pelleted and the pellets shock frozen in liquid N2. The cells were homogenized using VK05 glass beads (Precellys), and RNA was extracted using Qiagen's RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
The Affymetrix GeneChip 3' IVT PLUS Reagent Kit Protocol was used for the preparation of biotinylated aRNA from 150 ng of total RNA.
|
| |
|
| Hybridization protocol |
6 µg of fragmented a-RNA were hybridized for 16hr at 45C in a GeneChip hybridization oven 640.
|
| Scan protocol |
Arrays were scanned using an Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Summarization using RMA excluding all S. pombe probes was carried out using affy R package. Each temperature was processed separately with the control samples of the corresponding batch.
|
| |
|
| Submission date |
Jun 04, 2019 |
| Last update date |
Dec 25, 2019 |
| Contact name |
Evi Berchtold |
| E-mail(s) |
[email protected]
|
| Organization name |
Ludwig-Maximilians-University Munich
|
| Department |
Institute for Informatics
|
| Street address |
Amalienstr. 17
|
| City |
Munich |
| ZIP/Postal code |
80333 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2529 |
| Series (1) |
| GSE132186 |
Yeast heat shock response at 37C, 42C and 46C |
|
