|
| Status |
Public on Mar 09, 2010 |
| Title |
54_129_KO |
| Sample type |
RNA |
| |
|
| Source name |
temporal lobe
|
| Organism |
Mus musculus |
| Characteristics |
tissue: temporal lobe
strain: 129SV
genotype: WFS1 KO
|
| Treatment protocol |
Temporal lobes were dissected under dissection microscope
|
| Growth protocol |
Frozen in liquid nitrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1.2 ug total RNA (One-Cycle Target Labeling kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
Gene expression data from temporal lobe of wfs1 deficient mice
|
| Data processing |
Images were processed using the Affymetrix Microarray Suite 5.0, cel files were normalized with GCRMA and further analyzed using Bioconductir software based on R language
|
| |
|
| Submission date |
Mar 19, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Sulev Koks |
| E-mail(s) |
[email protected]
|
| Phone |
+61864570313
|
| Organization name |
Murdoch University
|
| Department |
Centre for Molecular Medicine and Innovative Therapeutics
|
| Street address |
90 South Street
|
| City |
Perth |
| State/province |
Western Australia |
| ZIP/Postal code |
6150 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15293 |
Gene expression profiling of temporal lobes of wfs1 deficient mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
