|
| Status |
Public on Mar 17, 2009 |
| Title |
Chronic Lymphocytic Leukemia (Unmutated) CLL UM 626 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
CLL UM 626
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Frozen Biopsy Specimen; Chronic Lymphocytic Leukemia (Unmutated)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exiqon
|
| Label |
Cy5
|
| Label protocol |
MiRNA expression profiling from human B cell lymphomas was conducted using up to 1 ug of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark).
|
| |
|
| Channel 2 |
| Source name |
Human Normal Lymph node total RNA (Ambion)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Normal lymph node
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exiqon
|
| Label |
Cy3
|
| Label protocol |
MiRNA expression profiling from human B cell lymphomas was conducted using up to 1 ug of total RNA from sample and reference (normal lymph node), which were labeled with Cy3 or Cy5 fluorescent dyes, using the miRNA/LNA labeling kit (Exiqon, Denmark).
|
| |
|
| |
| Hybridization protocol |
Exiqon protocol; The fluorescently labeled samples were combined and hybridized to a miRNA microarray (v.10.0, Exiqon, Denmark), in a nitrogen atmosphere.
|
| Scan protocol |
GenePix Pro 6.0.1.25. The microarray slides were scanned with GenePix 4100 Scanner.
|
| Description |
micro RNA expression
|
| Data processing |
The GPR file was extracted and exported to Genespring 7.3.1. The raw data were normalized using Data transformation: set measurements less than 0.01 to 0.01, per spot and per chip: Intensity dependent (Lowess) normalization, and per gene: normalize to median
|
| |
|
| Submission date |
Mar 16, 2009 |
| Last update date |
Nov 25, 2024 |
| Contact name |
Dereje Jima |
| E-mail(s) |
[email protected]
|
| Organization name |
NCSU
|
| Department |
CHHE
|
| Street address |
850 Main Campus Drive
|
| City |
RALEIGH |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
|
| Platform ID |
GPL7722 |
| Series (1) |
| GSE15250 |
Patterns of micro RNA expression characterize stages of human B-cell differentiation |
|
